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Figure 1: Purity of freshly-isolated microglia. CD11b+ cells freshly-isolated from perfused mouse brains as described were co-stained for CD45 and analyzed by flow cytometry. Only a small percentage of mouse brain cells were positive for both markers (left panel). Following magnetic isolation of CD11b+ cells from perfused brains, approximately 97% of the cells were positive for CD11b and CD45 (right panel).
Image Text (High Precision): CD45
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