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Figure 6: Day 7 A: Effect of the 5-HT2B receptor agonist BW723C86 in aorta from Sham-high salt, DOCA-high salt, DOCA-low salt and Sham rats. B: Effect of the 5-HT1B receptor agonist CP93129 in aorta from Sham-high salt, DOCA-high salt, DOCA-low salt and Sham rats. C: Effect of 5-HT in aorta from Sham-high salt, DOCA-high salt, DOCA-low salt and Sham rats. Data are reported as a percentage of the initial phenylephrine (PE) 10-5 M contraction. Points represent the mean ± sem for the number of experiments indicated in parentheses. * represents statistically significant difference (p < 0.05) between the response obtained in the Sham artery.

Image Text (High Precision): 10 5-HT BW723C86 DOCA Day M Sham contraction high salt

Other Images from "Arterial expression of 5-HT2B and 5-HT1B receptors during development of DOCA-salt hypertension":


Figure 8 Top: Measurement of 5-HT2B receptor prot...

Figure 7 Top: Measurement of 5-HT1B receptor prot...

Figure 9 Systolic blood pressures (SBP), EC50 val...

Figure 6 Day 7 A: Effect of the 5-HT2B receptor a...

Figure 5 Day 5 A: Effect of the 5-HT2B receptor a...

Figure 4 Day 3 A: Effect of the 5-HT2B receptor a...

Figure 3 Day 1 A: Effect of the 5-HT2B receptor a...

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Abstract

Background5-hydroxytryptamine (5-HT)2B and 5-HT1B receptors are upregulated in arteries from hypertensive DOCA-salt rats and directly by mineralocorticoids. We hypothesized that increased 5-HT2B and 5-HT1B receptor density and contractile function would precede increased blood pressure in DOCA-high salt rats. We performed DOCA-salt time course (days 1, 3, 5 and 7) studies using treatment groups of: DOCA-high salt, DOCA-low salt, Sham and Sham-high salt rats.ResultsIn isolated-tissue baths, DOCA-high salt aorta contracted to the 5-HT2B receptor agonist BW723C86 on day 1; Sham aorta did not contract. The 5-HT1B receptor agonist CP93129 had no effect in arteries from any group. On days 3, 5 and 7 CP93129 and BW723C86 contracted DOCA-high salt and Sham-high salt aorta; Sham and DOCA-low salt aorta did not respond. Western analysis of DOCA-high salt aortic homogenates revealed increased 5-HT2B receptor levels by day 3; 5-HT1B receptor density was unchanged. Aortic homogenates from the other groups showed unchanged 5-HT2B and 5-HT1B receptor levels.ConclusionThese data suggest that functional changes of 5-HT2B but not 5-HT1B receptors may play a role in the development of DOCA-salt hypertension.


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