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Figure 3.: Detection of citrullinated CII in human synovial fluid. (a) Immunoprecipitation of citrullinated CII from synovial fluid. Western blot staining with the antimodified citrulline antibody (AMC) upon appropriate pretreatment of the nitrocellulose membrane and immunostaining with the anticitrullinated CII mAb ACC2 are shown (+, ELISA-positive synovial fluid; −, ELISA-negative synovial fluid [compare with b]). Shown is a representative result of three independently performed experiments. (b) Capture ELISA for detection of citrullinated CII in synovial fluid specimens obtained from patients with OA, Reac A, and RA. Synovial fluid specimens were collected from patients with OA (n = 47), RA (n = 10), and Reac A (n = 15).
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Other Images from "Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis":
Figure 1. ACC mAb antibody specificity in vitro an...
Figure 2. Citrulline-specific antibodies mediate a...
Figure 3. Detection of citrullinated CII in human ...
Figure 4. Molecular structural analysis of the ACC...